Protective effect of breviscapine against brain injury induced by intrauterine inflammation in preterm rats and its mechanism / 中国当代儿科杂志

OBJECTIVES@#To study the protective effect of breviscapine against brain injury induced by intrauterine inflammation in preterm rats and its mechanism.@*METHODS@#A preterm rat model of brain injury caused by intrauterine inflammation was prepared by intraperitoneal injections of lipopolysaccharide in pregnant rats. The pregnant rats and preterm rats were respectively randomly divided into 5 groups: control, model, low-dose breviscapine (45 mg/kg), high-dose breviscapine (90 mg/kg), and high-dose breviscapine (90 mg/kg)+ML385 [a nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor, 30 mg/kg] (n=10 each). The number and body weight of the live offspring rats were measured for each group. Hematoxylin-eosin staining was used to observe the pathological morphology of the uterus and placenta of pregnant rats and the pathological morphology of the brain tissue of offspring rats. Immunofluorescent staining was used to measure the co-expression of ionized calcium binding adaptor molecule-1 (IBA-1) and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) in the cerebral cortex of offspring rats. ELISA was used to measure the levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-1β (IL-1β) in the brain tissue of offspring rats. Western blotting was used to measure the expression of Nrf2 pathway-related proteins in the brain tissue of offspring rats.@*RESULTS@#Pathological injury was found in the uterus, and placenta tissue of the pregnant rats and the brain tissue of the offspring rats, and severe microglia pyroptosis occurred in the cerebral cortex of the offspring rats in the model group. Compared with the control group, the model group had significant reductions in the number and body weight of the live offspring rats and the protein expression levels of Nrf2 and heme oxygenase-1 (HO-1) in the brain tissue of the offspring rats (P<0.05), but significant increases in the relative fluorescence intensity of the co-expression of IBA-1 and NLRP3, the levels of the inflammatory factors IL-6, IL-8, and IL-1β, and the protein expression levels of NLRP3 and caspase-1 in the brain tissue of the offspring rats (P<0.05). Compared with the model group, the breviscapine administration groups showed alleviated pathological injury of the uterus and placenta tissue of the pregnant rats and the brain tissue of the offspring rats, significant increases in the number and body weight of the live offspring rats and the protein expression levels of Nrf2 and HO-1 in the brain tissue of the offspring rats (P<0.05), and significant reductions in the relative fluorescence intensity of the co-expression of IBA-1 and NLRP3, the levels of the inflammatory factors IL-6, IL-8, and IL-1β, and the protein expression levels of NLRP3 and caspase-1 in the brain tissue of the offspring rats (P<0.05). The high-dose breviscapine group had a significantly better effect than the low-dose breviscapine (P<0.05). ML385 significantly inhibited the intervention effect of high-dose breviscapine (P<0.05).@*CONCLUSIONS@#Breviscapine can inhibit inflammatory response in brain tissue of preterm rats caused by intrauterine inflammation by activating the Nrf2 pathway, and it can also inhibit microglial pyroptosis and alleviate brain injury.

Clinical Research of Qingkailing Injection in Adjuvant Treatment of Sepsis and Heat Syndrome in Children / 中国实验方剂学杂志

Objective:To evaluate the clinical efficacy of Qingkailing injection in the treatment of children with sepsis and heat syndrome， and investigate its anti-inflammatory， anticoagulant and protective effects. Method:Eighty patients were randomly divided into control group and observation group with forty cases in each group according to the number table. Both groups received comprehensive treatment measures such as fluid resuscitation， anti-infection， anti-inflammatory， anticoagulation， vasoactive drugs， and protection of vital organ functions. While patients in observation group additionally took Qingkailing injection， 5-10 mL each time， intravenous drip after dilution， 1 time/day. Treatment course was five days in both groups. Before and after treatment， the scores of quick sequential organ failure assessment （qSOFA）， pediatric critical illness score （PCIS） and acute physiology and chronic health evalution Ⅱ （APACHEⅡ） were graded； procalcitonin （PCT）， serum amyloid A protein （SAA） before and after treatment， heparin-binding protein （HBP）， tumor necrosis factor-<italic>α</italic> （TNF-<italic>α</italic>）， high-sensitivity C-reactive protein （hs-CRP）， interleukin-6 （IL-6）， IL-10， N-terminal brain sodium Peptide precursor （NT-proBNP）， high-sensitivity cardiac troponin T （hs-cTnT） level， cardiac troponin I （cTnI）， creatine kinase isoenzyme （CK-MB）， <italic>D</italic>-dimer （<italic>D-</italic>D ）， fibrinogen （FIB） and antithrombin Ⅲ （AT-Ⅲ） levels were detected. Result:The APACHEⅡ and qSOFA scores in the observation group were lower than those in the control group （<italic>P</italic><0.05）， while the PCIS score was higher than that in the control group （<italic>P</italic><0.05）. The levels of PCT， SAA，HBP，TNF-<italic>α</italic>， hs-CRP and IL-6 in the observation group were lower than those in the control group （<italic>P</italic><0.01）， while the IL-10 level was higher than that in the control group （<italic>P</italic><0.01）. The levels of NT-proBNP， hs-cTnT， cTnI， CK-MB， <italic>D</italic>-D and FIB in the observation group were lower than those in the control group （<italic>P</italic><0.01）， while the AT-Ⅲ activity was higher than that in the control group （<italic>P</italic><0.01）. Conclusion:Qingkailing injection as the adjuvant therapy in children with sepsis and fever syndrome， can play the role of anti-inflammatory， anticoagulant， reducing infection and myocardial damage， thereby reducing the severity of the disease and improving the prognosis.

Relationship between Polymorphism in ALOX5, ALOX5AP and Susceptibility to Myeloid Leukemia / 中国实验血液学杂志

OBJECTIVE@#To investigate the correlation of single nucleotide polymorphisms (SNP) in arachidonate 5-lipoxygenase gene (ALOX5) rs2029253, rs2228064 and rs2228065 sites, 5-lipoxygenase activating protein gene (ALOX5AP) rs10507391, rs4769874 sites with the risk for genesis of adult myeloid leukemia.@*METHODS@#By the approval from the hospital ethics committee and the informed consent of participants. 150 patients with myeloid leukemia (ML) as ML group and 134 healthy people as the control group were selected. The genomic DNA was extracted from the samples. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with directly sequencing, PCR-amplified products were applied to test the polymorphism of 5 sites in ALOX5 and ALOX5AP gene.@*RESULTS@#A allele frequencies of ALOX5 gene rs2029253 site in the ML group and the control group were 43.0% and 34.3%, respectively. And the G allele frequencies in the ML group and the control group were 57.0% and 65.7%, respectively. The genotype distributions of AA, AG and GG in ALOX5 gene rs2029253 site in the ML group were 32.2%, 21.5% and 46.3% respectively. That in the control group were 15.7%, 37.3% and 47.0% respectively. The genotype AA and A allele frequency of ALOX5 gene rs2029253 site were linked with the increased risk of myeloid leukemia (OR=2.26, 95% CI: 1.43-4.56, P＜0.05; OR=1.44, 95% CI: 1.02-2.03, P＜0.05). And the genotype AG and allele G reduced the susceptibility to myeloid leukemia (OR=0.46, 95% CI: 0.27-0.78, P＜0.01; OR=0.69, 95% CI: 0.50-0.98, P＜0.05), however, the polymorphisms of ALOX5 gene rs2228064 and rs2228065 site not correlated with the risk of myeloid leukemia (P＞0.05). The A allele frequency of ALOX5AP gene rs10507391 site in the ML group and the control group were 30.7% and 36.2% respectirely. The genotype distribution rates of AA, AT and TT in ALOX5AP gene rs10507391 site in the ML group was 1.3%, 58.7% and 40.0% respectively, that in the control group were 9.7%, 53.0% and 37.3% respectively. The genotype AA of ALOX5AP gene rs10507391 site correlated with the decreased risk of myeloid leukemia (OR=0.13, 95% CI: 0.03-0.57, P＜0.05), but the polymorphism of ALOX5AP gene rs4769874 site not correlated with the risk of myeloid leukemia (P＞0.05).@*CONCLUSION@#The genotype AA, AG and allele A, G of ALOX5 rs2029253, as well as ALOX5AP rs10507391 may be correlate with the susceptibility to myeloid leukemia.

Related factors of cognitive behavior therapy for adults with attention-deficit/hyperactivity disorder / 中国心理卫生杂志

Objective:To explore the related factors to the effect of cognitive behavior therapy (CBT) for adults with attention-deficit/hyperactivity disorder(ADHD).Methods:A prospective and open-label,self-control study was designed.Eighty-five adult patients from outpatient department with ADHD diagnosed with the Diagnostic and Statistical Manual of Mental Disorders,Fourth Edition(DSM-Ⅳ).All the patients received 12 weeks of CBT,the ADHD Rating Scale (ADHD-RS) was used to evaluate ADHD core symptoms,and according to the post-treat ADHD-RS reduction ratio,41 patients were divided into responders(ADHD-RS reduction ratio≥25％)and 44 patients were divided into non-responders(ADHD-RS reduction ratio ＜ 25％).The t-test and chi-square test were used to analyze the differences of sociodemographic variables,clinical variables and early efficacy (ADHD-RS reduction ratio≥25％ in the fourth week) between the two groups,and logistics regression was used to analysis the related factors.Results:Comparing with the group of non-responders,there was more cases with early efficacy (P ＜ 0.05)in the group of responders.Logistic models indicated that higher scores of BIS-motor (OR =1.27),early efficacy (OR =11.87) were associated with better CBT efficacy.Conclusion:It suggests that ADHD adults with more sever motor impulsiveness symptom,and early efficacy may get favorable response to CBT.

Sex differences in resting-state fractional amplitude of low frequency fluctuation of functional magnetic resonance imaging in children with attention-deficit/hyperactivity disorder / 中国心理卫生杂志

Objective:To find out the difference between children with attention-deficit/hyperactivity disorder and normal control,and the difference between boys and girls with ADHD in resting-state.Methods:There were 38 children with ADHD in this study including 31 boys and 7 girls,meanwhile,42 normal controls whose age and gen der were matched with children with ADHD were involved too,including 29 boys and 13 girls.Total scores of ADHD rating scale (ADHD-RS) were used to measure the severity of symptom for both ADHD and normal control.Magnetic resonance imaging was used to collect the resting-state functional magnetic resonance imaging data,and the fractional amplitude of low frequency fluctuation (fALFF) was measured for each child.Analysis of covariance (ANCOVA) was used to find out the difference between the group and the sex.Results:Children with ADHD showed a higher fALFF in the right medial superior frontal gyrus and right supplementary motor area,and a lower fALFF in the left putamen,bilateral cerebellum and bilateral precuneus than controls (all corrected P ＜0.05).ADHD boys showed a lower fALFF in the left cerebellum and a higher fALFF in the bilateral medial superior frontal gyrus than boys without ADHD (all corrected P ＜0.05).ADHD girls showed a higher fALFF in the bilateral cerebellum and in the thalamus than girls without ADHD,and a lower fALFF in the insula than girls without ADHD (all corrected P ＜ 0.05).There was a negative relationship between the fALFF of the left cerebellum of ADHD girls and the scores of inattention symptoms.Conclusion:The fALFF difference exist between ADHD and normal controls,and between boys and girls with ADHD.

Seminal plasma anti-Müllerian hormone and inhibin B and serum inhibin B in predicting the outcome of routine IVF fertilization / 中华男科学杂志

Objective@#To analyze the correlations of seminal plasma (sp) anti-Müllerian hormone (spAMH) and inhibin B (spINHB) and serum INHB (serINHB) with semen parameters in oligoasthenospermia patients and explore their value in predicting the outcome of routine in vitro fertilization (IVF).@*METHODS@#We obtained the levels of spAMH, spINHB and serINHB as well as semen parameters from 88 infertile males undergoing IVF due to oligoasthenospermia or female uterine tubal factors from August 2016 to February 2017. Using the ROC curve and Pearson's correlation analysis, we examined the effects of the obtained parameters on the fertilization rate and assessed the correlation of the levels of spAMH, spINHB and serINHB with the semen parameters of the patients.@*RESULTS@#Concerning the predictive value for the outcome of IVF, Pearson's correlation analysis showed that the area under the ROC curve (AUC) of spAMH was 0.807 (sensitivity = 84.6%, specificity = 76%, cut-off point = 3.529, P <0.001) and that of spINHB was 0.768 (sensitivity = 84.6%, specificity = 88.7%, cut-off point = 31.117, P = 0.002). The serINHB level was found positively correlated with sperm concentration (r = 0.346, P = 0.001), total sperm count (r = 0.378, P <0.001), sperm motility (r = 0.521, P <0.001), and the percentage of progressively motile sperm (r = 0.343, P = 0.001).@*CONCLUSIONS@#The levels of spAMH and spINHB can be used as laboratory indexes to predict the fertilization rate of routine IVF and are correlated with semen parameters in oligoasthenospermia patients, while that of serINHB has a positive correlation with the semen parameters of the patients.

Synthesis and cytotoxicity of longistylin C derivatives / 中国天然药物

The present study was designed to identify potent anti-tumor compounds from a series of new longistylin C derivatives. Ten longistylin C derivatives were synthesized and their structures were confirmed by (1)H NMR, MS, and elemental analyses. Their cytotoxicity in vitro against three human cancer cell lines (A549, HepG2, and MCF-7) were evaluated by the MTT assay. Among these compounds, DT-6 and DT-9 displayed much better cytotoxicity against A549, HepG2, and MCF-7 cells, DT-1 exhibited selective cytotoxicity against HepG2, and the structure-activity relationships were investigated. In conclusion, Compounds DT-6 and DT-9 may serve as potential lead compounds for the discovery of new anti-cancer drugs.

Study on the AIDS-related high risk behaviors of men who have sex with men under the experience of exchanging money for the same sex / 中华流行病学杂志

Objective To investigate the AIDS-related high risk behaviors of men who havesex with men (MSM) who ever exchanged money for the same sex. Methods Target sampling forcross-sectional study and valid anonymous questionnaires were adopted to compare the differences ofAIDS-related high risk behaviors between MSM with the experience of exchanging money for thesame sex and those without that experience. 1959 rstadied samples were recruited in nine cities. SPSS13.0 was used and t, x 2 and Mann-Whitney tests were taken for statistical analysis. ResultsCompared to corresponding ones without "buying" the same sex experience, MSM who ever engagedin "buying" sex had the characteristics of being prone to in marriage and living in larger cities witholder age and higher income (P＜0.01). They also had a significant larger number in the followingevents: total sexual panners, anal sex and oral sex episodes with same sex, number of sexualpartners, anal sex and oral sex in the previous six months, with the figure of median 50.0, 20.0,20.0, 5.0,4.0,5.0 respectively. 31.5% had ever participated in 'group sex', 48.0% had sex with malepartners away from his own region in the previous year, 70.5% had sexual intercourse withstrangers at MSM avenues in the last six months. The OR (95% CI) values were 2.288( 1.702-3.077), 3.231 (2.462-4.241 ), 2.840 (2.140-3.770). All the above mentioned figures werehigher than those without the experience, with significant differences. They had a significant lower rate of 45.7% of condom use while having sex with female partners (P＜0.05). Conclusion MSM with the experience of exchanging money for the same sex would have more AIDS-related high risk behaviors, AIDS preventative measures should be taken to target different subgroups.

Inhibition of hepatitis B virus (HBV) replication using antisense LNA targeting to both S and C genes in HBV / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To investigate the inhibitory effect on HBV replication of antisense locked nucleic acid (LNA) targeting to both S and C genes in HBV transgenic mice.</p><p><b>METHODS</b>Thirty HBV transgenic mice were randomly divided into five groups (n = 6): glucose control group were treated with 5% glucose solution, liposome control group were treated with liposome alone, S group were treated with LNA targeting to S gene, C group were treated with LNA targeting to C gene, and dual-target group were treated with LNA targeting to both S and C genes. Antisense LNA was injected into mice via the tail vein. Serum HBsAg was quantified by TRFIA. Serum HBV DNA was quantified by real-time PCR. The expression of HBV C-mRNA in the liver was detected by RT-PCR. The expression of HBsAg and HBcAg in the liver was detected by immunohistochemistry. Serum ALB, ALT, BUN and CR were measured using an automatic biochemical analyzer. The effects of antisense LNA on mouse organs were investigated by HE staining.</p><p><b>RESULTS</b>5 days after LNA injection, serum HBsAg levels in the dual-target group were reduced by 72.8%, and serum HBV DNA levels were decreased by 52.9%. These values were significantly higher than those in the control groups (all P < 0.05). No significant differences were noted in serum ALB, ALT, BUN and CR between the experiment groups and the control groups (all P > 0.05). The expression levels of HBsAg and HBcAg in the liver of dual-target group were significantly lower than those in the control groups. No significant histopathological abnormality was found in liver and kidney tissues in all groups.</p><p><b>CONCLUSION</b>Antisense LNA targeting to both S and C genes can significantly inhibit HBV replication in transgenic mice.</p>

Study on HIV/AIDS related high risk behaviors among unmarried men who have sex with women / 中华流行病学杂志

Objective To explore the risk of sexual behaviors related to AIDS among unmarried men who often have sex with women.Methods Study objectives were recruited through target sampling and anonymous questionnaires were adopted.Men' s sexual behaviors were compared with each other between the group of men who always had sex with women (group A) and who never had sex with women (group B) in the past 6 months.Results The average numbers of same-gender partners among group A who acted as the male role in the past 6 months and who had anal intercourse in the last month were 5.64 and 3.09 respectively,which were both higher than those of group B's (P＜ 0.05).The rate of condom use among men in group A when having sex with men was 81.94% .The percentages of condom use among men in group A who having had oral or anal sex with male sex buyers last time were 15.38% and 70.27% respectively.They were all higher than those of group B' s (P＜0.05 or 0.01 ).The rates of men in group A who were male sex workers and who having had sex with unknown men in gay places during the past 6 months were 27.59% and 61.70% respectively.Among group A,the percentages of men who had had sex with unknown men during the last time and who had had sex with nonlocal male partners during the past year were 43.06% and 40.28% respectively.They were all higher than those of group B's (P＜0.01).The percentage of condom use among men in group A when having had sex with women during the past 6 months was 68.94%,but only 25.96% of the men used condom regularly.The rate of condom use in men from group A when having sex with women during the last sexual intercourse was 65.45%.Conclusion HIV high-risk sex behaviors were more ubiquitous among unmarried MSM who always had sex with women,suggesting that different interventions be developed to prevent them from transmitting HIV from MSM to women or other men.

Study on the high risk behaviors related to AIDS among men who having sex with men in the floating population / 中华流行病学杂志

Objective To explore the risks on sexual behaviors related to AIDS among men who having sex with men (MSM) in floating population and to develop relevant interventions. Methods Study objectives were recruited through target sampling and anonymous questionnaires were adopted. Male sexual behaviors were compared between floating population and permanent registered residents.Results Among those MSM, the average numbers of same-gender partners, oral sex, anal sex and sex with females during the past 6 months among floating population were 9.61, 8.61, 7.52 and 1.17 respectively. Numbers of partners on oral sex and anal sex during the past month were 2.89 and 3.69 respectively. Both figures were higher than those of the permanent registered residents (P<0.05 or P< 0.01). The rates of condom use among floating population when having anal sex with men or with non-marital female partners were 37.15% and 23.28% respectively. The figures were all higher than those of permanent registered residents' (P<0.05 or P<0.01). Among floating population, the percentages of men who had sex with male sex workers or who had sex with men acquainted through inter-net or with stable female sex partners were 17.89%, 12.56%, and 31.86% respectively. The rates of men who had group sex among gay men, who bled when having sex with men or who had sex with men from other areas during the past year were 22.13%, 31.38% and 32.36% respectively. The figures were all higher than those of permanent registered residents' (P<0.05 or P<0.01). Conclusion Sex-related high risk behaviors were more ubiquitous among floating population than those of permanent registered residents because they were more likely to transmit HIV to people from/to other areas. More attention should be paid to the floating population with MSM in particular, when health interventions are carried out.

Effects of nitric oxide and hydrogen sulfide on the relaxation of pulmonary arteries in rats / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The balance between vasodilation and vasoconstriction plays a major role in maintaining vascular homeostasis. However, the underlying mechanisms are unclear. More and more evidence suggested that there was an interaction in the regulation of vasorelaxation between nitric oxide (NO) and hydrogen sulfide (H(2)S). We explored the interaction between and effects of NO and H(2)S on the relaxation of pulmonary arteries in rats.</p><p><b>METHODS</b>Seven male Sprague-Dawley rats were anaesthetized with chloral hydrate and the pulmonary arteries of each rat separated for the study of vascular activities. The vasorelaxing activities of pulmonary artery rings in response to different doses of a NO donor, sodium nitroprusside (SNP), or a H(2)S donor, sodium hydrogen sulfide (NaHS), were measured in vitro. When pulmonary artery rings were treated with a cystathionine-gamma-lyase inhibitor, DL-propargylglycine, in the presence of SNP or a nitric oxide synthase inhibitor, N(omega)-nitro-L-arginine methyl ester, in the presence of NaHS, the changes in relaxing activities were analyzed.</p><p><b>RESULTS</b>The relaxation of pulmonary artery rings was in a dose dependent manner in response to either SNP or NaHS. The relaxation rates of pulmonary artery rings increased from (30.90+/-4.62)% to (60.50+/-8.08)% when the concentration of SNP increased from 1 micromol/L to 3 micromol/L and from (26.13+/-4.12)% to (53.09+/-14.01)% when the concentration of NaHS increased from 25 micromol/L to 100 micromol/L. However, when appropriate inhibitor was added, the relaxation responses to SNP and NaHS decreased.</p><p><b>CONCLUSIONS</b>The results suggested that similarly to NO, H(2)S acted as a vasorelaxant either independently of, or synergistically with NO in the regulation of vasorelaxation. The interaction between NO and H(2)S played an important role in regulating relaxing activities of pulmonary arteries.</p>

Associations between partner-seeking activities through internet and sexual risk behaviours related to HIV/AIDS among men who have sex with men in China / 中华流行病学杂志

Objective To explore the associations between partner-seeking activities through internet and HIV/AIDS related risk behaviours among men who have sex with men(MSM).Methods Target sampling.anonymous questionnaires were adopted and analyzed with SPSS 11.0.Results Data from 45.6% of the 2178 valid questionnaires showed that most partner-seeking activities was through internet.73.1% of them landed on gay website.in which 83.0% looked for sexual partners and 91.7% of them had intercourse with other men met through internet when compared with MSM who did not seek sex activities through internet.The internet group obviously had fewer numbers of partners(5.73 vs.10.3),oral sex partners(5.13 vs.8.71),passive anus sex partners(4.67 vs.8.26)and active anus sex partners (3.51 vs.6.06)in the past 6 months(P＜0.01 respectively)and obviously had fewer group sex(16.7% vs.20.5%)activities in the past one year but had more activities on oral sex(94.7% vs.89.6%),anus sex(95.1% vs.86.5%),regular sexual partner(52.6% vs.46.7%)in the past 6 months(83.4% vs.75.5%)(P＜0.01 respectively)and more likely to have finger intercourse(52.2% vs.38.5%),hemorrhage sexual intercourse(36.4% vs.21.9%,OR=2.04),non-local sexual intercourse(28.1% vs.22.7%)(P＜0.01 respectively).Conclusion Seeking sexual partners through internet had positive and double effects on MSM's risk behaviours.suggesting that it is of urgent need to take the advange of internet and gay website to disseminate HIV/AIDS prevention message.

Effect of epigallocatechingallate on acute lung injury induced by oleic acid in mice / 药学学报

<p><b>AIM</b>To investigate the effect of epigallocatechingallate (EGCG) on acute lung injury induced by oleic acid in mice and the possible mechanism.</p><p><b>METHODS</b>Acute lung injury was induced by oleic acid in mice. Light microscopy and electron microscopy were used to examine histological changes and lung index as well as wet to dry weight ratio was calculated. Serum TNF-a level was measured by enzyme linked immunosorbent assay (ELISA) and the phosphorylation of p38 MAPK was determined by Western blotting.</p><p><b>RESULTS</b>Pretreatment of EGCG significantly alleviated oleic acid induced lung injury accompanied by reduction of lung index and wet to dry weight ratio, decreased of TNF-a level in serum and inhibition of phosphorylation of p38 MAPK.</p><p><b>CONCLUSION</b>EGCG showed beneficial effect on acute lung injury induced by oleic acid in mice. The ultimate reduction of TNF-alpha in serum caused by inhibition of phosphorylated p38 MAPK is involved in the mechanism of action of EGCG.</p>

Effects of Ligustrazine on Secretion of Nitric Oxide in Ventricular Tissues of Rats under Physiological Condition / 实用儿科临床杂志

Objective To explore the effects of ligustrazine on secretion of nitric oxide(NO) in ventricular tissues of rats under physiologi-cal condition.Methods Twenty-four normal wistar rats were divided into three groups,eight each group.The left and right ventricular tissues of each rats were divided into control group and 2 ligustrazine groups and the final concentrations of ligustrazine were 15 mg/L and 150 mg/L respectively.The ventricular tissues that had been cutting into small pieces were incubated in 37 ℃ thermostatic water bath for 4 hours with Krebs solution as supernatant.After incubation of the ventricular tissues,the spectrophotometric method was used to detect the content of NO_2~-/NO_3~-in the supernatant.Results Compared with the content of NO in control group,it was significantly decreased in the 150 mg/L ligustrazine group(P

Changes of Endogenous Hydrogen Sulfide in Plasma of Newborn Infant with Pulmonary Hypertension / 实用儿科临床杂志

Objective To explore the changes of hydrogen sulfide(H2S) levels in plasma of newborn infants with pulmonary hypertension(PH) and its relationship with pulmonary hypertension,and provide scientific evidence for the decision of treating neonatal PH.Methods Sixteen children with PH and 16 children without PH in ICU from Mar.2005 to Mar.2006 were selected.Ultrasonic cardiogram(UCG) examination was performed for eachpatients.Pulmonary artery pressure(PAP) was measured.The plasma concentrations of H2S,cysteine and PAP of each patient were measured.Results PAP was 4.27-9.73 kPa[(6.49?1.79) kPa] in neonatal PH group,and PAP in control group was normal.The plasma levels of cysteine and H2S in neonatal PH group significantly increased compared with those of control group [(11.94?6.65) ?mol/L vs(6.43?2.08) ?mol/L,t=2.630 P=0.016;(26.99?1.33) ?mol/L vs(24.92?1.36) ?mol/L,t=4.373 P=0].Conclusions Endogenous H2S and cysteine were up-regulated during the development of neonatal PH;it might play an improtant role in the development of PH.H2S possibly depress the PAP by dilating the pulmonary artery to protect the patients with pulmonary hy pertension.

Separation and purification of active fraction with macroporous resigns / 中成药

AIM: To purify the water-extract of Sinisa recipe with macoroporous resigns. METHODS: Screening the purification condition by means of the content of active principle, fingerprint chromatography, the yield of dry extracts,et al. of the purified product. RESULTS: The purified product by type HP20 macoroporous resign is in good agreement with the active fraction of Sinisa in the composition of specific principles. CONCLUSION: HP20 type macoroporos resign is considered as optimum for active fraction of Sinisa recipe in purifying efficience.

Influence of Carbon Monoxide on the Content of Atrial Natriuretic Peptide in Ventricular Tissues of Rats / 实用儿科临床杂志

Objective To investigate the influence of carbon monoxide(CO) on the content of atrial natriuretic peptide(ANP) in ventricular tissues of rats.Methods The left and right ventricular tissues of 12 Wistar rats were divided into control group(n=12) and hemin group(n=12,the final concentration of hemin was 10~(-4) mol/L),respectively.The ventricular tissues were successly incubated in(37 ℃) thermostatic waterbath with Krebs solution as supernatant for 4 hours.After incubation of the ventricular tissues,radio-immunity method was applied to determine the content of ANP in the supernatant.Results Compared with the content of ANP in the supernatant in control group,it was significantly decreased both in left [(9.72?3.59) vs(52.05?31.65) ng/(g?wet tissue),P